crLiga de Ascenso (2. liga)

Club from the Aserrí canton near San José, founded in 1940, one of the competition's oldest participants.

Club from the city of Cariari in Limón province, promoted to the Liga de Ascenso after winning the third division in 2019/20.

Club from the Barrio El Carmen district of Alajuela, founded in 1948, playing at Estadio Rafael Bolaños.

Club from the city of Palmares (Alajuela province), founded in 2012, promoted to the second division after just three seasons in 2015.

Club from the city of Naranjo (Alajuela province), founded in 2020, playing at the Estadio Municipal de Naranjo.

Club from the artisan town of Sarchí, famous for its traditional hand-painted oxcarts.

Club from the city of Coronado near San José, founded in 1936, historic league champion in 1963.

Club from the Moravia canton, led since the 2026/27 season by former basketball player and TV commentator Mónica Malavassi.

Club from the Guadalupe district in the Goicoechea canton, relegated to the second division after a season in the top flight.

Club from the village of Jicaral on the Nicoya Peninsula, full name Jicaral Sercoba de la Península, Liga de Ascenso runner-up in 2024.

Club from the city of Grecia (Alajuela province) nicknamed "the Greek Panthers", playing at the Estadio Allen Riggioni Suárez.

Club from the city of Santa Ana (San José province), won promotion to the top flight in 2024 before returning to the second division.

New club from Santa Bárbara (Heredia province), promoted to the Liga de Ascenso as champions of the lower LINAFA Second B division.

Club from the Pacific coast port town of Quepos, founded in 1996.

Club from the city of Santa Cruz in Guanacaste, a franchise belonging to Nicaragua's Real Estelí, originally operating under the name PFA Antioquia.